our publications

2010

Bryan, N.B., Dorfleutner, A., Kramer, S.J., Yun, C., Rojanasakul, Y. and Stehlik, C. (2010) Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes. J. Inflammation., in press.

2009

Stehlik, C. (2009) Multiple proteases are responsible for IL-1β maturation in inflammatory arthritis. Arthritis Rheum., 60, 3524-3530.

Bryan, N.B., Dorfleutner, A., Rojanasakul, Y, and Stehlik, C. (2009) Activation of inflammasomes requires intracellular redistribution of the apoptotic speck-like protein containing a caspase recruitment domain (ASC). J. Immunol., 182, 3173-3182. - featured in The Jourmal of Immunology "in this issue" as one of the top articles

2008

Dorfleutner, A, Chi, Y., Vincent, D., Cunnick, J.M., Lin, H., Weed, S.A., Stehlik, C., and Flynn, D.C. (2008) Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells. J. Cell Sci., 121, 2394-2405.

Wang; L., Chanvorachote, P., Toledo, D., Stehlik, C., Mercer, R.R., Castranova, V. and Rojanasakul, Y. (2008) Peroxide is a Key Mediator of Bcl-2 Downregulation and Apoptosis Induction by Cisplatin in Human Lung Cancer Cells. Mol. Pharm., 73, 119-127.

2007

Stehlik, C. and Dorfleutner, A. (2007) COPs & POPs: Modulators of Inflammasome Activity. J. Immunol., 179, 7993-7998.

Dorfleutner, A., Stehlik, C., Zhang, J., Gallick, G.E., Flynn, D.C. (2007) AFAP-110 is required for actin stress fiber formation and cell adhesion in MDA-MB-231 breast cancer cells. J. Cell. Physiol., 213, 740-749.

Stehlik, C. (2007) The PYRIN domain in signal transduction. Curr. Prot. Pept. Sci., 8, 293-310.

Dorfleutner, A., Bryan, N.B., Talbott, S.J., Fynya, K.N., Rellick, S.L., Reed, J.C., Shi, X., Royanasakul, Y., Flynn, D.C., and Stehlik, C. (2007) Cellular PYRIN domain-only protein (cPOP) 2 is a novel regulator of inflammasome activation. Infect. Immun., 75, 1484-1492. - featured as a Spotlight article in Infection and Immunity.

Dorfleutner, A., Talbott, S.J., Bryan, N.B., Funya, K.N., Rellick, S.L., Reed, J.C., Shi, X., Flynn, D.C, Rojanasakul, Y. and Stehlik, C. (2007) A shope Fibroma virus PYRIN-only protein modulates the host immune response. Virus Genes., 35, 685-694.

2006

Azad, N., Chanvorachote, P., Nimmanit, U., Stehlik, C., Ongpipatanakul, B., and Rojanasakul, Y. (2006). Reactive Oxygen Species-mediated S-Nitrosylation and stabilization of Bcl-2. J. Biol.Chem., 281, 34124-34134.

Lu, B., Wang, L., Stehlik, C., Medan, D., Huang, C., Hu, S., Chen, F., Shi, X., and Rojanasakul, Y. (2006) Phosphatidylinositol 3-Kinase/Akt Positively Regulates Fas (CD95)-Mediated Apoptosis in Epidermal Cl41 Cells. J. Immunol. 176, 6785-6793.

Chanvorachote, P., Nimmanit, U., Stehlik, C., Ongpipatanakul, B., and Rojanasakul, Y. (2006). Nitric Oxide Regulates Cell Sensitivity to Cisplatin-Induced Apoptosis through S-Nitrosylation and Inhibition of Bcl-2 Ubiquitination. Cancer Res. 66, 6353-6360.

2005

Chanvorachote, P., Nimmanit, U., Wang, L., Stehlik, C., Lu, B., Azad, N., and Rojanasakul, Y. (2005) Nitric oxide negatively regulates Fas (CD95)-induced apoptosis through inhibition of ubiquitin-proteasome mediated degradation of FLIP. J. Biol. Chem. 280, 42044-42050.

Medan, D., Wang, L., Toledo, D., Lu, B., Stehlik, C., Shi, X., and Rojanasakul, Y. (2005) Regulation of Fas (CD95)-mediated apoptotic and necrotic cell death by reactive oxygen species in macrophages. J. Cell Physiol. 203,78-84.

2004

Bruey, J.M., Bruey-Sedano, N., Newman, R., Chandler, S., Stehlik, C., and Reed, J.C.  (2004) PAN1/NALP2/PYPAF2, an inducible inflammatory mediator that regulates NF-kappaB and caspase-1 activation in macrophages. J. Biol. Chem. 279, 51897-51907.

Kroismayr, R., Baranyi, U., Stehlik, C., Dorfleutner, A., Binder, B.R., and Lipp, J. (2004) HERC5, a HECT E3 ubiquitin ligase tightly regulated in LPS activiated endothelial cells. J. Cell Science 117, 4749-4756.

Stehlik C., Kroismayr, R., Dorfleutner A., Binder B.R., and Lipp J. (2004) VIGR – a novel inducible adhesion family G-protein coupled receptor in endothelial cells. FEBS Letters 569, 149-155. supplemental information

*Stehlik, C. and Reed, J.C. (2004) The PYRIN Connection: Novel Players in innate immunity and inflammation. (Review) J. Exp. Med. 200, 551-558. *Corresponding Author

2003

Stehlik, C., Lee, S.H., Dorfleutner A., Stassinopoulos, A., Sagara, J., and Reed, J.C. (2003) Apoptosis-associated speck-like protein containing a caspase recruitment domain is a Regulator of pro-Caspase-1 Activation, J. Immunol. 171, 6154-63.

Reed, J.C., Doctor, K., Rojas, A., Zapata, J.M., Stehlik, C., Fiorentino, L., Damiano, J., Roth, W., Matsuzawa, S., Newman, R., Takayama, S., Marusawa, H., Xu, F., Salvesen, G., and Godzik, A. (2003) Comparative Analysis of Apoptosis and Inflammation Genes of Mice and Humans. Genome Res.13(6B), 1376-1388.

Stehlik, C., Hayashi, H., Pio, F., Godzik, A., and Reed, J.C. (2003) CARD6, a CARD containing inhibitor of Nod1 and Cardiak induced NF-kB activation. J. Biol. Chem. 278, 31941-31949.

Stehlik, C., Krajewska, M., Welsh, K., Krajewski, S., Godzik, A., and Reed, J.C. (2003) The PAAD/PYRIN-only protein POP is a modulator of ASC-mediated NF-kB and pro-Caspase-1 regulation. Biochem. J. 372, 101-113. - editorial: Mariathasan, S., and Vucic, D. (2003) POPing the fire into the pyrin? Biochem. J. 372, e1-2.

2002

Stehlik, C., Fiorentino, L., Dorfleutner,A., Bruey, J.M., Ariza, E.M., Sagara, J., and Reed, J.C. (2002) The PAAD/PYRIN-family protein ASC is a dual regulator of a conserved step in NF-kB activation pathways. J. Exp. Med. 196,1605-1615.

*Fiorentino, L., *Stehlik, C. Oliveira, V., Ariza, M.E., Godzik, A., and Reed, J.C. (2002) A Novel PAAD-containing Protein That Modulates NF-kB Induction by Cytokines Tumor Necrosis Factor-a and Interleukin-1b. J. Biol. Chem. 277, 35333-35340. *equally contributed

2001

*Damiano, J.S., *Stehlik, C., Pio, F., Godzik, A., and Reed, J.C. (2001) CLAN, a novel human CED4 like gene. Genomics 75, 77-83. *equally contributed

Lee S.H., Stehlik C., Reed J.C. (2001) COP, a CARD-containing protein and inhibitor of caspase-1 activation processing. J. Biol. Chem. 276, 34495-34500.

2000

Hofer-Warbinek, R., Schmid, J.A., Stehlik, C., Binder, B.R., Lipp, J. and de Martin, R. (2000). Activation of NF-kappaB by XIAP, the X chromosome-linked inhibitor of apoptosis. J. Biol. Chem. 275, 22064-22068.

1998

Stehlik, C., de Martin, R., Kumabashiri, I., Schmid, J.A., Binder, B.R. and Lipp, J. (1998). Nuclear Factor (NF)- kB-regulated X-chromosome-linked iap Gene Expression Protects Endothelial Cells from Tumor Necrosis Factor a-induced Apoptosis. J. Exp. Med. 188, 211-216.

Stehlik, C., de Martin, R., Binder, B.R. and Lipp, J. (1998). Cytokine induced expression of porcine Inhibitor of Apoptosis Protein (iap) family member is regulated by NF-kB. Biochem. Biophys. Res. Com. 234, 827-832.